Part:BBa_K1922009

hTert-GFP

Introduction

Since the telomerase is activated in almost cancer cells and we hope hTert promoter can improve the targeting in thermotherapy. To test if the promoter can help the overexpression of selected gene, we make it linked with a GFP. So when we transfect the plasmid in telomerase positive and negative cells, different results can be observed, which means we can make sure the promoter is working, when GFP is seen in cancer cells instead of negative ones.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 263
  Illegal BsaI.rc site found at 1415
  

Construction

PCR:
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Function

Our part require hTERT promoter to reach specifically targeting, so we have designed experiment to illustrate that the part works as we expected. hTERT-GFP was overexpressed in both breast cancer cell hcc 1937 and telomerase-negative cells. Observed the cell in fluorescent microscope directly so we could see that the hTERT prompter is able to make the downstream gene transcripted in tumor cells specifically.
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